Day: June 18, 2024

The post EXPERT ANALYSIS: Why is Crimea the one place where Ukraine is Winning? appeared first on The Cipher Brief.

‘Don’t Have More to Add…’: US Refrains From Commenting on Pannun Murder Plot  Republic World

Fact check: Trump falsely claims almost all new jobs under Biden have gone to ‘illegal aliens’  CNN

Megachurch pastor and ex-Trump adviser admits child sexual abuse  The Guardian US

Kansas Attorney General Kris Kobach sues Pfizer for COVID-19 vaccine  WIBW

During the COVID-19 pandemic, the detection and sequencing of SARS-CoV-2 from wastewater proved to be a valuable tool in assessing trends at the community level. Several whole genome enrichment methods have been proposed for sequencing SARS-CoV-2 from the mixed wastewater community, but there is little consensus on the most appropriate sequencing methods for variant detection or abundance estimations. Few studies have elucidated the errors associated with these methods or have established minimum sequencing requirements for correct interpretation of the results. To address these needs, we systematically assessed the efficacy of three tiled amplicon enrichment methods (Freed/Midnight, ARTIC V4, NEB VarSkip) for whole genome sequencing of SARS-CoV-2 variants using mock wastewater communities with variants at known proportions. We found the ARTIC V4 approach yielded the most accurate results for variant identification and variant abundance estimation, followed by the NEB VarSkip approach. Conversely, the NEB VarSkip method obtained the highest genomic coverage, with the ARTIC V4 method achieving the second highest coverage. Finally, we determined that the Freed/Midnight library preparation methods are not well-suited for use with short read sequencing. Based on the present results, the ARTIC V4 workflow appears to be the most robust and cost-effective approach for monitoring circulating SARS-CoV-2 variants with wastewater surveillance.

Putin Just Proved Trump’s Peace Plan Is a Total Joke  Yahoo! Voices

Potential of a Trump return inspires Biden and G7 rush to guarantee long-term support for Ukraine  CNN

Gan & Lee Pharmaceuticals to Present Groundbreaking Data on Three Innovative Products at the American Diabetes …  PR Newswire

How a new campaign spending law could boost controversial North Carolina Trump ally Mark Robinson  USA TODAY