The post A new COVID variant is on the rise in the US. What are its most common symptoms? – TODAY first appeared on The CoronaVirus Alerts – The News And Times.
Day: July 4, 2024
Recent years have seen intense interest in the development of point-of-care nucleic acid diagnostic technologies to address the scaling limitations of laboratory-based approaches. Chief among these are combinations of isothermal amplification approaches with CRISPR-based detection and readouts of target products. Here, we contribute to the growing body of rapid, programmable point-of-care pathogen tests by developing and optimizing a one-pot NASBA-Cas13a nucleic acid detection assay. This test uses the isothermal amplification technique NASBA to amplify target viral nucleic acids, followed by Cas13a-based detection of amplified sequences. We first demonstrate an in-house formulation of NASBA that enables optimization of individual NASBA components. We then present design rules for NASBA primer sets and LbuCas13a guide RNAs for fast and sensitive detection of SARS-CoV-2 viral RNA fragments, resulting in 20 – 200 aM sensitivity without any specialized equipment. Finally, we explore the combination of high-throughput assay condition screening with mechanistic ordinary differential equation modeling of the reaction scheme to gain a deeper understanding of the NASBA-Cas13a system. This work presents a framework for developing a mechanistic understanding of reaction performance and optimization that uses both experiments and modeling, which we anticipate will be useful in developing future nucleic acid detection technologies.
The post Developing, characterizing and modelingCRISPR-based point-of-use pathogen diagnostics first appeared on The CoronaVirus Alerts – The News And Times.
Prosecutors in classified files case to urge judge to bar Trump from inflammatory comments about FBI AM 870 The ANSWER
The post Prosecutors in classified files case to urge judge to bar Trump from inflammatory comments about FBI – AM 870 The ANSWER first appeared on The Trump Investigations – trumpinvestigations.net – The News And Times.
Governor Pillen Provides Further Guidance Regarding Special Session to Fix Property Tax Crisis | Office of Governor … Governor Pete Ricketts
The post Governor Pillen Provides Further Guidance Regarding Special Session to Fix Property Tax Crisis | Office of Governor … – Governor Pete Ricketts first appeared on The Trump Investigations – trumpinvestigations.net – The News And Times.
(Повтор сообщения от 2 июля)
The post От экономики до здравоохранения: следующее правительство Британии ждет гора проблем first appeared on The News And Times.
The post От экономики до здравоохранения: следующее правительство Британии ждет гора проблем first appeared on Trump And The FBI – The News And Times.
АЛМА-АТА, 4 июл (Рейтер) – Остатки на корреспондентских
счетах кредитных организаций в Национальном банке Казахстана на
3 июля выросли до 618,005 миллиарда тенге с 577,254 миллиарда
тенге ранее, сообщил ЦБ.
Ниже следуют данные ЦБ на отчетную дату:
счетах кредитных организаций в Национальном банке Казахстана на
3 июля выросли до 618,005 миллиарда тенге с 577,254 миллиарда
тенге ранее, сообщил ЦБ.
Ниже следуют данные ЦБ на отчетную дату:
объем в
млрд тенге
03.07.2024 618,005
02.07.2024 577,254
01.07.2024 831,377
28.06.2024 665,412
27.06.2024 689,069
26.06.2024 624,267
25.06.2024 921,310
24.06.2024 642,140
21.06.2024 637,607
20.06.2024 595,900
19.06.2024 835,775
18.06.2024 706,651
17.06.2024 557,828
14.06.2024 558,560
13.06.2024 541,457
12.06.2024 510,033
11.06.2024 546,443
10.06.2024 533,463
07.06.2024 595,768
06.06.2024 549,750
05.06.2024 581,376
04.06.2024 540,255
03.06.2024 764,514
31.05.2024 711,719
30.05.2024 615,700
29.05.2024 672,125
28.05.2024 627,745
27.05.2024 475,096
24.05.2024 572,560
23.05.2024 616,338
22.05.2024 668,696
21.05.2024 512,770
20.05.2024 564,117
17.05.2024 646,993
16.05.2024 520,908
15.05.2024 583,443
14.05.2024 515,525
13.05.2024 707,532
10.05.2024 557,633
06.05.2024 726,842
04.05.2024 574,764
03.05.2024 532,609
02.05.2024 574,504
30.04.2024 563,135
29.04.2024 607,151
26.04.2024 623,087
25.04.2024 590,431
24.04.2024 519,034
23.04.2024 549,707
22.04.2024 544,724
19.04.2024 524,982
18.04.2024 681,256
17.04.2024 592,580
16.04.2024 604,787
15.04.2024 620,247
12.04.2024 507,640
11.04.2024 484,542
10.04.2024 485,448
09.04.2024 550,608
08.04.2024 485,813
05.04.2024 605,490
04.04.2024 546,853
03.04.2024 628,863
02.04.2024 682,820
01.04.2024 554,005
29.03.2024 574,750
28.03.2024 562,620
27.03.2024 581,756
26.03.2024 586,742
20.03.2024 586,161
19.03.2024 588,142
18.03.2024 540,406
15.03.2024 586,090
14.03.2024 528,358
13.03.2024 604,468
12.03.2024 525,671
11.03.2024 571,492
07.03.2024 596,105
06.03.2024 732,202
05.03.2024 769,880
04.03.2024 771,091
01.03.2024 634,004
29.02.2024 720,903
28.02.2024 660,185
27.02.2024 609,262
26.02.2024 628,523
23.02.2024 479,627
22.02.2024 458,131
21.02.2024 528,043
20.02.2024 494,965
19.02.2024 541,127
16.02.2024 494,422
15.02.2024 459,661
14.02.2024 550,450
13.02.2024 554,810
12.02.2024 534,567
09.02.2024 542,379
08.02.2024 596,955
07.02.2024 720,332
06.02.2024 703,933
05.02.2024 717,751
02.02.2024 669,660
01.02.2024 676,980
31.01.2024 674,672
30.01.2024 734,065
29.01.2024 689,281
26.01.2024 781,411
25.01.2024 578,834
24.01.2024 582,821
23.01.2024 612,799
22.01.2024 605,056
19.01.2024 552,163
18.01.2024 570,257
17.01.2024 576,309
16.01.2024 585,000
15.01.2024 552,325
14.01.2024 544,381
11.01.2024 517,123
10.01.2024
591,483
09.01.2024 517,843
08.01.2024 511,617
05.01.2024 514,685
04.01.2024 511,623
03.01.2024 740,715
29.12.2023 978,508
28.12.2023 836,217
27.12.2023 682,170
26.12.2023 684,464
25.12.2023 573,336
22.12.2023 634,912
21.12.2023 611,179
20.12.2023 568,236
19.12.2023 676,718
15.12.2023 594,343
14.12.2023 604,398
13.12.2023 650,616
12.12.2023 536,361
11.12.2023 613,252
08.12.2023 669,854
07.12.2023 583,212
06.12.2023 615,607
05.12.2023 817,052
04.12.2023 656,851
01.12.2023 588,955
(Мария Гордеева)
The post Остатки на корсчетах в Нацбанке Казахстана на 3 июл выросли до 618,005 млрд тенге first appeared on The News And Times.
The post Остатки на корсчетах в Нацбанке Казахстана на 3 июл выросли до 618,005 млрд тенге first appeared on Trump And The FBI – The News And Times.
