Day: June 18, 2024
During the COVID-19 pandemic, the detection and sequencing of SARS-CoV-2 from wastewater proved to be a valuable tool in assessing trends at the community level. Several whole genome enrichment methods have been proposed for sequencing SARS-CoV-2 from the mixed wastewater community, but there is little consensus on the most appropriate sequencing methods for variant detection or abundance estimations. Few studies have elucidated the errors associated with these methods or have established minimum sequencing requirements for correct interpretation of the results. To address these needs, we systematically assessed the efficacy of three tiled amplicon enrichment methods (Freed/Midnight, ARTIC V4, NEB VarSkip) for whole genome sequencing of SARS-CoV-2 variants using mock wastewater communities with variants at known proportions. We found the ARTIC V4 approach yielded the most accurate results for variant identification and variant abundance estimation, followed by the NEB VarSkip approach. Conversely, the NEB VarSkip method obtained the highest genomic coverage, with the ARTIC V4 method achieving the second highest coverage. Finally, we determined that the Freed/Midnight library preparation methods are not well-suited for use with short read sequencing. Based on the present results, the ARTIC V4 workflow appears to be the most robust and cost-effective approach for monitoring circulating SARS-CoV-2 variants with wastewater surveillance.
The post Comparison of three tiled amplicon sequencing approaches for SARS-CoV-2 variant detection from wastewater first appeared on The CoronaVirus Alerts – The News And Times.
Potential of a Trump return inspires Biden and G7 rush to guarantee long-term support for Ukraine CNN
The post Potential of a Trump return inspires Biden and G7 rush to guarantee long-term support for Ukraine – CNN first appeared on The Trump Investigations – trumpinvestigations.net – The News And Times.
How a new campaign spending law could boost controversial North Carolina Trump ally Mark Robinson USA TODAY
The post How a new campaign spending law could boost controversial North Carolina Trump ally Mark Robinson – USA TODAY first appeared on The Trump Investigations – trumpinvestigations.net – The News And Times.
По словам местных жителей, пожар вспыхнул после удара дрона на заводе по производству метанола в Азове.
The post Под Ростовом из-за атаки дронов загорелись нефтяные резервуары first appeared on The News And Times.
The post Под Ростовом из-за атаки дронов загорелись нефтяные резервуары first appeared on Trump And The FBI – The News And Times.
По данным нового замминистра обороны РФ Анны Цивилевой, у каждого пятого военного, вернувшегося с войны, выявляют посттравматический синдром. При этом психологов для работы с ними не хватает.
The post Замминистра обороны РФ: Посттравматический стресс у 20 процентов вернувшихся с войны first appeared on The News And Times.
The post Замминистра обороны РФ: Посттравматический стресс у 20 процентов вернувшихся с войны first appeared on Trump And The FBI – The News And Times.
SEOUL, South Korea — South Korean soldiers on Tuesday fired warning shots to repel North Korean soldiers who temporarily crossed the rivals’ land border for the second time this month, South Korea’s military said.
South Korea’s Joint Chiefs of Staff said around 20 to 30 North Korean soldiers, while engaging in unspecified construction work on the northern side of the border, briefly crossed the military demarcation line that bisects the countries as of 8:30 a.m. It said the North Korean soldiers retreated after the South broadcasts warnings and fired warning shots and the South’s military didn’t spot any suspicious activities after that.
The South also fired warning shots on June 11 after another group of North Korean soldiers briefly crossed the MDL. The Joint Chiefs of Staff said that Tuesday’s incident occurred in a different area along the central frontline region. It said it doesn’t believe the North Korean soldiers intruded the border intentionally and that the North did not return fire.
The South’s military said it has been observing increased North Korean construction activity in frontline border areas, such as installing suspected anti-tank barriers, reinforcing roads and planting land mines.
The border intrusions come as tensions rise between the war-divided rivals, who in recent weeks have engaged in Cold War-style psychological warfare and made it clear they are no longer bound by their landmark military agreement in 2018 to reduce tensions.
The post South Korean soldiers fire warning shots after North Korean troops intrude for 2nd time this month first appeared on The News And Times.
The post South Korean soldiers fire warning shots after North Korean troops intrude for 2nd time this month first appeared on Trump And The FBI – The News And Times.
